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Aliquots of 100–200 mg were dissected with a forceps and subsequently ground to fine powder in liquid nitrogen in a grinder mill (Retch). CTAB buffer (2% CTAB, 2% PVP, 20 mM EDTA, 100 mM Tris–HCl, pH, M NaCl, 2% 2mercaptoethanol) was added to 100 mg of powdered sample and incubated for 2 h at 65°C under agitation.
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Lactose fermentation by Kombucha – a process to obtain new milk–based beverages Article (PDF Available) in Romanian Biotechnological Letters 17(1) · January 2012 with 1,083 Reads